Control of functional mRNA stability in bacteria: multiple mechanisms of nucleolytic and non-nucleolytic inactivation
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چکیده
منابع مشابه
Catalysis by the nucleolytic ribozymes.
The nucleolytic ribozymes use general acid-base catalysis to contribute significantly to their rate enhancement. The VS (Varkud satellite) ribozyme uses a guanine and an adenine nucleobase as general base and acid respectively in the cleavage reaction. The hairpin ribozyme is probably closely similar, while the remaining nucleolytic ribozymes provide some interesting contrasts.
متن کاملVisualizing the structure and mechanism of a small nucleolytic ribozyme.
Time-resolved crystallography has recently evolved into a powerful and invaluable technique for observing conformational and chemical intermediate states at or near atomic resolution in protein enzymes. The application of monochromatic time-resolved X-ray crystallographic freeze-trapping experiments to an RNA enzyme, the hammerhead ribozyme, is outlined here.
متن کاملHuman telomerase catalyzes nucleolytic primer cleavage.
Telomerase is a reverse transcriptase that uses an integral RNA molecule to add de novo G-rich repeats onto telomeric DNA, or onto nontelomeric DNA generated during chromosome fragmentation and breakage events. A telomerase-mediated DNA substrate cleavage activity has been reported in ciliates and yeasts. Nucleolytic cleavage may serve a proofreading function, enhance processivity or ensure tha...
متن کاملNon-involvement of nucleolytic activities in the early effect of dimethylnitrosamine on the content of free mRNA in mouse liver.
1. After dimethylnitrosamine (DMNA) administration to mice, the content of poly(A)-containing RNA decreases rapidly in the postmicrosomal fraction of the liver. We report here that the loss of free mRNA is not a result of increased nucleolytic activity. On the contrary, a decreased activity of microsomal endonuclease, assayed by its effect on polyribosomal mRNA, was demonstrated already 15 min ...
متن کامل[Cytochemical localization and properties of selected nucleolytic enzymes].
In the article there are shortly outlined studies on cytochemical localization of selected nucleolytic enzymes carried out between 1957-1986 by David Shugar and his coworkers. The histochemical localization of several nucleolytic enzymes in animal and plant tissues was determined by synthesis of specific substrates, alpha-naphthyl esters of 5'- and 3'-nucleotides and their derivatives. In rat t...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 1992
ISSN: 0950-382X
DOI: 10.1111/j.1365-2958.1992.tb01469.x